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| Line 2: |
Line 2: |
| | __NOTOC__ | | __NOTOC__ |
| | <partinfo>BBa_K3011007 short</partinfo> | | <partinfo>BBa_K3011007 short</partinfo> |
| − |
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| − | Constitutive Promoter (BBa_J23100) – RBS (BBa_B0032) – NsrR (BBa_K1682011) – Terminator (BBa_B0010) – PyeaR promoter (BBa_K216005) – RBS (BBa_B0032) – mRFP (BBa_E1010) – Terminator (BBa_B0012)
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| − |
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| − | NsrR (BBa_K1682011) is a Nitric Oxide (NO) sensitive transcription repressor. This construct produces NsrR constitutively; in absence of NO, NsrR binds to the NsrR binding site present on the PyeaR promoter (BBa_K216005) and repress the transcription of mRFP (BBa_E1010). In presence of NO, NsrR binds to NO due to which NsrR can’t bind to the DNA (thus can’t repress gene expression) and mRFP will produce.
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| − |
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| − | NsrR contains a Helix Turn Helix (HTH) motif and Fe-S cluster ([2Fe-2S] or [4Fe-4S]). In absence of NO, NsrR binds to a particular DNA sequence by using HTH motif (as a homodimer) and repress gene expression. In presence of NO, Fe-S cluster reacts with NO to form dinitrosyl iron complex, due to the formation of this complex NsrR can’t bind to that particular sequence anymore. Depending upon the sequence of NsrR binding site, the number of NO molecules (per cluster) which is required for abolishing DNA binding vary (2-10 NO / cluster)
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