Difference between revisions of "Part:BBa K3011007:Design"
 
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__NOTOC__
 
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<partinfo>BBa_K3011007 short</partinfo>
 
<partinfo>BBa_K3011007 short</partinfo>
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NorR (BBa_K256004) is a Nitric Oxide (NO) dependent transcription activator. This construct produces NorR constitutively; in presence of NO, NorR can activate the transcription of mRFP (BBa_E1010) which is under control of pNO promoter (BBa_K256000). 
 
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NorR contains a Helix Turn Helix (HTH) motif by which it can bind to a particular DNA sequence (as a homodimer). In presence of NO it can activate transcription of genes which is under control of pNO promoter. The regulatory domain of NorR contains a mononuclear non-haem iron centre (Fig. 2.1), which reversibly binds NO. Binding of NO stimulates the ATPase activity of NorR, enabling the activation of transcription.
 
  
  

Latest revision as of 08:21, 20 October 2019


Nitric Oxide sensor


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 202
    Illegal AgeI site found at 1222
    Illegal AgeI site found at 1334
  • 1000
    COMPATIBLE WITH RFC[1000]