Difference between revisions of "Part:BBa K103003"
 
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<partinfo>BBa_K103003 short</partinfo>
 
<partinfo>BBa_K103003 short</partinfo>
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B domain of Staphylococcal protein A, which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of Z<sub>SPA-1</sub>.
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B domain of Staphylococcal protein A[http://www.ncbi.nlm.nih.gov/pubmed/7236608?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum], which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of Z<sub>SPA-1</sub>.
  
 
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Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]
 
===Usage and Biology===
 
===Usage and Biology===
 
*Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
 
*Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
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*We have exploited it's ability of binding Z<sub>SPA-1</sub> protein to create our 'hunter and prey system'
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*We have used this protein as hunter fused with [[Part:BBa_K103017 | BBa_K103017]] or [[Part:BBa_K103018 | BBa_K103018]] in our 'hunter and prey' selection system
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*This part uses our BamHI/SacI/NdeI fusion cloning substandard
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| [[Image:Protein_a.jpg]]
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source: [http://www.pdb.org/pdb/explore.do?structureId=1BDD PDB]
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Latest revision as of 00:53, 28 October 2008

B domain of Staphylococcal protein A

B domain of Staphylococcal protein A[http://www.ncbi.nlm.nih.gov/pubmed/7236608?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum], which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of ZSPA-1.


Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]

Usage and Biology

  • Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
  • We have used this protein as hunter fused with BBa_K103017 or BBa_K103018 in our 'hunter and prey' selection system
  • This part uses our BamHI/SacI/NdeI fusion cloning substandard
 Protein a.jpg

source: [http://www.pdb.org/pdb/explore.do?structureId=1BDD PDB]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 91
    Illegal BamHI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]