Difference between revisions of "Part:BBa K3165039"
 
(2 intermediate revisions by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3165039 short</partinfo>
 
<partinfo>BBa_K3165039 short</partinfo>
  
−
This part consists of the mini-CcaS protein complex with Gp2 protein under the pCpcG2 promoter.
+
This part consists of the mini-CcaS protein complex <html><a  href="https://parts.igem.org/Part:BBa_K3165037">(BBa_K3165037)</a></html> with Gp2 protein <html><a  href="https://parts.igem.org/Part:BBa_K3165038">(BBa_K316508)</a></html> under the PcpcG2 promoter <html><a  href="https://parts.igem.org/Part:BBa_K2012015">(BBa_K2012015)</a></html>.
  
  
Line 12: Line 11:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
The mini-CcaS protein consisting of a GAF domain, two PAS domains and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. Gp2 placed downstream of the pCpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS.
+
The mini-CcaS protein consisting of a GAF domain, two PAS domains and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. Gp2 placed downstream of the PcpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS.
 
This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.
 
This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.
  

Latest revision as of 19:04, 21 October 2019

mini-CcaS + Gp2

This part consists of the mini-CcaS protein complex (BBa_K3165037) with Gp2 protein (BBa_K316508) under the PcpcG2 promoter (BBa_K2012015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The mini-CcaS protein consisting of a GAF domain, two PAS domains and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. Gp2 placed downstream of the PcpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS. This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.