Difference between revisions of "Part:BBa K3165036"
 
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<partinfo>BBa_K3165036 short</partinfo>
 
<partinfo>BBa_K3165036 short</partinfo>
  
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This part comprises of the phycocyanobilin production unit [heme oxygenase 1 (Ho1) and phycocyanobilin ferredoxin oxidoreductase (PcyA)] and the CcaR protein.
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This part comprises of the phycocyanobilin production unit Heme oxygenase 1 <html><a  href="https://parts.igem.org/Part:BBa_K3165002">(Ho1)</a></html> and phycocyanobilin ferredoxin oxidoreductase <html><a  href="https://parts.igem.org/Part:BBa_K3165003">(PcyA)</a></html> and the CcaR <html><a  href="https://parts.igem.org/Part:BBa_K3165001">(CcaR)</a></html> protein.
  
  
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<h2> Biology </h2>
 
<h2> Biology </h2>
  
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The enzymes required for the production of phycocyanobilins [heme oxygenase 1 (Ho1) and phycocyanobilin ferredoxin oxidoreductase (PcyA)] are encoded by the part along with the response regulator (CcaR) of the CcaSR system. The production of phycocyanobilins and the CcaR protein are both crucial for the functioning of the green light-sensitive CcaS-CcaR system.
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The enzymes required for the production of phycocyanobilin Heme oxygenase 1 <html><a  href="https://parts.igem.org/Part:BBa_K3165002">(Ho1)</a></html> and phycocyanobilin ferredoxin oxidoreductase <html><a  href="https://parts.igem.org/Part:BBa_K3165003">(PcyA)</a></html> are encoded by the part along with the response regulator <html><a  href="https://parts.igem.org/Part:BBa_K3165001">(CcaR)</a></html> of the CcaSR system. The production of phycocyanobilins and the CcaR protein are both crucial for the functioning of the green light-sensitive CcaS-CcaR system.
  
 
<h2> Usage </h2>
 
<h2> Usage </h2>

Latest revision as of 01:26, 21 October 2019


Ho1 + PcyA + CcaR

This part comprises of the phycocyanobilin production unit Heme oxygenase 1 (Ho1) and phycocyanobilin ferredoxin oxidoreductase (PcyA) and the CcaR (CcaR) protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1758
    Illegal NheI site found at 1781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

The enzymes required for the production of phycocyanobilin Heme oxygenase 1 (Ho1) and phycocyanobilin ferredoxin oxidoreductase (PcyA) are encoded by the part along with the response regulator (CcaR) of the CcaSR system. The production of phycocyanobilins and the CcaR protein are both crucial for the functioning of the green light-sensitive CcaS-CcaR system.

Usage

This part encodes for two indispensable components of the optogenetic CcaS-CcaR system. This part can be used along with the CcaS encoding sequence to establish the complete optogenetic system.