Difference between revisions of "Part:BBa K2957097"
 
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<partinfo>BBa_K2957017 short</partinfo>
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<partinfo>BBa_K2957099 short</partinfo>
  
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Contents: CMV, CCL5, Flag tag, SynthpA, with Carb resistance
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<b>Contents:</b> CMV, CCL5 with Flag tag, SynthpA, with Carb resistance
  
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What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression.  
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<b>What is it?:</b> A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression.  
  
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What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a Flag tag attached.  
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<b>What does it do?:</b> It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a Flag tag attached.  
  
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "
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<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2957017 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2957099 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2957017 parameters</partinfo>
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<partinfo>BBa_K2957099 parameters</partinfo>
 
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Latest revision as of 00:28, 20 October 2019


hEF1a, IL-8 (CXCL-8)

Contents: CMV, CCL5 with Flag tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and CCL5 gene allowing for constitutive CCL5 expression.

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a Flag tag attached.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2684
    Illegal BamHI site found at 3290
    Illegal BamHI site found at 3569
    Illegal XhoI site found at 3083
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
    Illegal NgoMIV site found at 197
    Illegal NgoMIV site found at 2818
    Illegal AgeI site found at 2025
    Illegal AgeI site found at 2196
    Illegal AgeI site found at 3673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2073
    Illegal SapI.rc site found at 9