Difference between revisions of "Part:BBa K2957016"

Latest revision as of 00:20, 20 October 2019

hEF1a, CCL5-Flag

Contents: hEF1a, CCL5 with Flag tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and CCL5 gene allowing for constitutive CCL5 expression.

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a Flag tag attached.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1089
Illegal SpeI site found at 92
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 92
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1089
Illegal SpeI site found at 92
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1089
Illegal SpeI site found at 92
Illegal AgeI site found at 1095
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 <b>What does it do?:</b> It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a Flag tag attached.
-<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "
+<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
 <!-- Add more about the biology of this part here