Difference between revisions of "Part:BBa K3165034"
 
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<h2> Biology </h2>
 
<h2> Biology </h2>
  
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Heme Oxygenase 1 catalyzes the oxidative degradation of heme into biliverdin IX alpha, which is the first step in the formation of phycocyanobilins. Further, phycocyanobilin ferredoxin oxidoreductase catalyzes the four-electron reduction of the biliverdin IX alpha formed in the former step in to phycocyanobilins (PCBs). This part provides the composite machinery for the biosynthesis of Phycocyanobilins.
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Heme Oxygenase 1 <html><a href="https://parts.igem.org/Part:BBa_K3165030">(Ho1)</a></html>
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catalyzes the oxidative degradation of heme into biliverdin IX alpha, which is the first step in the formation of phycocyanobilins. Further, phycocyanobilin ferredoxin oxidoreductase <html><a href="https://parts.igem.org/Part:BBa_K3165058">(PcyA)</a></html> catalyzes the four-electron reduction of the biliverdin IX alpha formed in the former step into phycocyanobilins (PCBs). This part provides the composite machinery for the biosynthesis of Phycocyanobilins.
  
 
<h2> Usage </h2>
 
<h2> Usage </h2>

Latest revision as of 00:47, 21 October 2019


Ho1 + PcyA

This part is used to generate the two enzymes responsible for the biosynthesis of phycocyanobilins (heme oxygenase and phycocyanobilin ferredoxin oxidoreductase).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

Heme Oxygenase 1 (Ho1) catalyzes the oxidative degradation of heme into biliverdin IX alpha, which is the first step in the formation of phycocyanobilins. Further, phycocyanobilin ferredoxin oxidoreductase (PcyA) catalyzes the four-electron reduction of the biliverdin IX alpha formed in the former step into phycocyanobilins (PCBs). This part provides the composite machinery for the biosynthesis of Phycocyanobilins.

Usage

This part is used for the synthesis of phycocyanobilin. In certain photo-sensitive sytems (like the CcaSR system), the production of phycocyanobilin is essential for the activity of the light-sensitive components.