Difference between revisions of "Part:BBa K2957014"
 
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<partinfo>BBa_K2957099 short</partinfo>
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<partinfo>BBa_K2957014 short</partinfo>
  
 
<b>Contents:</b> hEF1a, CCL5, SynthpA, with Carb resistance
 
<b>Contents:</b> hEF1a, CCL5, SynthpA, with Carb resistance
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<b>What does it do?:</b> It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.   
 
<b>What does it do?:</b> It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.   
  
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<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
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<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.  
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2957099 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2957014 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2957099 parameters</partinfo>
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<partinfo>BBa_K2957014 parameters</partinfo>
 
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Latest revision as of 23:59, 19 October 2019


hEF1a, CCL5

Contents: hEF1a, CCL5, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and CCL5 gene allowing for constitutive CCL5 expression

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of CCL5. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 535
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 535
    Illegal AgeI site found at 81
    Illegal AgeI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]