Difference between revisions of "Part:BBa K2924021:Design"
 
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===Design Notes===
 
===Design Notes===
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The acc genes were cloned in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.
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The genes from <i>E. Coli K12</i> were cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.
  
  
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===Source===
 
===Source===
  
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The genes were cloned from the genome of <i>E. coli</i>
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The genes were cloned from the genome of <i>E. coli K12</i>
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===References===
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Latest revision as of 19:49, 20 October 2019


accABCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 668
    Illegal AgeI site found at 1501
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 803


Design Notes

The genes from E. Coli K12 were cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.


Source

The genes were cloned from the genome of E. coli K12