Difference between revisions of "Part:BBa K3009012"
 
(4 intermediate revisions by the same user not shown)
Line 5: Line 5:
 
Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter.
 
Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter.
 
The aspartate racemase is consequently converting L-aspartate to D-aspartate.
 
The aspartate racemase is consequently converting L-aspartate to D-aspartate.
 +
 +
 +
We purified it via his-tag purification to perform further experiments.
 +
 +
<html>
 +
<figure>
 +
<img style="width:50%;" src="https://2019.igem.org/wiki/images/f/fc/T--Freiburg--racemase_purification.png">
 +
<figcaption>
 +
<b> Fig.2:</b> His-Tag Purification of <i>P.horikoshii</i> aspartate racemase.
 +
            Bands are clearly visible at 40 kDA.
 +
</figcaption>
 +
</figure>
 +
</html>
  
  

Latest revision as of 21:01, 20 October 2019


Aspartate Racemase (PhAspR)

Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. The aspartate racemase is consequently converting L-aspartate to D-aspartate.


We purified it via his-tag purification to perform further experiments.

Fig.2: His-Tag Purification of P.horikoshii aspartate racemase. Bands are clearly visible at 40 kDA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]