Difference between revisions of "Part:BBa K3142013"
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what it is&#65306;
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AcmA is an Endo-βN-acetylmuramidase that dissociates the reducing group of N-acetylmuramic acid from Gram-positive bacteria to autolyze the lactic acid bacteria.
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what it does&#65306;
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how to use it in their projects&#65306;
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==SZPT-China==
 
==SZPT-China==
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To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.  
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The results of ''LAB'' MG1363 and ''LAB'' MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 1. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.  
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[[file:p-a.png|center|500px]]
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[[file:Ａｃｍａａ.png|center|500px]]
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===Reference===
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*Coolbear, T., Pillidge, C. J., & Crow, V. L. (1994). The diversity of potential cheese ripeningcharacteristics of ''lactic acid starter bacteria'': Resistance to cell lysis and levels of cellular distribution of proteinase activities. International Dairy Journal, 4,697–721.

Latest revision as of 02:57, 22 October 2019


acmA

AcmA is an Endo-βN-acetylmuramidase that dissociates the reducing group of N-acetylmuramic acid from Gram-positive bacteria to autolyze the lactic acid bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 579
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


SZPT-China

The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 1. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.

Ａｃｍａａ.png

Reference

  • Coolbear, T., Pillidge, C. J., & Crow, V. L. (1994). The diversity of potential cheese ripeningcharacteristics of lactic acid starter bacteria: Resistance to cell lysis and levels of cellular distribution of proteinase activities. International Dairy Journal, 4,697–721.