Difference between revisions of "Part:BBa K3165032"

Latest revision as of 00:38, 21 October 2019

sRBS + CcaR v2.0

This part incorporates the CcaR encoding gene under a strong ribosome binding site sRBS (250).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

The CcaR encoding sequence is attached to a strong ribosome binding site sRBS (250) for the constitutive production of the CcaR protein in the cell under normal metabolic conditions.

Usage

This part is used as an essential component of the green light-sensitive CcaSR system. In association with the BioBrick encoding for the CcaS protein the green light-inducible protein expression system is established.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3165032 short</partinfo>
-This part incorporates the CcaR encoding gene under a strong ribosome binding site [sRBS(250)].
+This part incorporates the CcaR encoding gene under a strong ribosome binding site <html><a href="https://parts.igem.org/Part:BBa_K3165004">sRBS (250)</a></html>.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><b>Sequence and Features</b></span>
 <partinfo>BBa_K3165032 SequenceAndFeatures</partinfo>
+===Usage and Biology===
+<h2> Biology </h2>
+The CcaR encoding sequence is attached to a strong ribosome binding site <html><a href="https://parts.igem.org/Part:BBa_K3165004">sRBS (250)</a></html> for the constitutive production of the CcaR protein in the cell under normal metabolic conditions.
+<h3> Usage </h3>
+This part is used as an essential component of the green light-sensitive CcaSR system. In association with the BioBrick encoding for the CcaS protein the green light-inducible protein expression system is established.
 <!-- Uncomment this to enable Functional Parameter display