Difference between revisions of "Part:BBa K2984069"
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| − | The PETase is an enzyme that was discovered in the bacterium Ideonella sakaiensis and catalyzes the hydrolysis of PET to MHET (Yoshida et al. 2016). This enzyme has caught the attention of many research groups around the world for its potential to be used for biotechnological applications regarding PET degradation. | + | The PETase is an enzyme that was discovered in the bacterium <i>Ideonella sakaiensis</i> and catalyzes the hydrolysis of PET to MHET (Yoshida et al. 2016). This enzyme has caught the attention of many research groups around the world for its potential to be used for biotechnological applications regarding PET degradation. |
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Latest revision as of 23:15, 19 October 2019
PETase CDS; Enzyme that catalyzes the first degradation step of PET
The PETase is an enzyme that was discovered in the bacterium Ideonella sakaiensis and catalyzes the hydrolysis of PET to MHET (Yoshida et al. 2016). This enzyme has caught the attention of many research groups around the world for its potential to be used for biotechnological applications regarding PET degradation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 861
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 400
Illegal PstI site found at 861 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 124
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 861
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 861
- 1000COMPATIBLE WITH RFC[1000]
MoClo Overhangs
This part with B3-B3 MoClo overhangs can be found here.
This part with B2-B4 MoClo overhangs can be found here.
References
- Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., ... & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199.