Difference between revisions of "Part:BBa K2984068"
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<partinfo>BBa_K2984068 short</partinfo> | <partinfo>BBa_K2984068 short</partinfo> | ||
| − | Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for C. reinhardtii | + | Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for <i>C. reinhardtii</i>. |
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===MoClo Overhangs=== | ===MoClo Overhangs=== | ||
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| − | This part with A1-A3 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">here</a>. | + | <p>This part with A1-A3 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">here</a>.</p> |
| + | <p>This part with A1-B1 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984025">here</a>. | ||
| + | </p> | ||
</html> | </html> | ||
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<partinfo>BBa_K2984068 parameters</partinfo> | <partinfo>BBa_K2984068 parameters</partinfo> | ||
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| + | ==References== | ||
| + | <ol> | ||
| + | <li> | ||
| + | Schroda, M., Blöcker, D., & Beck, C. F. (2000). The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. The plant journal, 21(2), 121-131. | ||
| + | </li> | ||
| + | </ol> | ||
Latest revision as of 23:14, 19 October 2019
AR Promoter
Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for C. reinhardtii.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 264
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
MoClo Overhangs
This part with A1-A3 MoClo overhangs can be found here.
This part with A1-B1 MoClo overhangs can be found here.
References
- Schroda, M., Blöcker, D., & Beck, C. F. (2000). The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. The plant journal, 21(2), 121-131.