Difference between revisions of "Part:BBa K112400:Design"
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<partinfo>BBa_K112400 short</partinfo> | <partinfo>BBa_K112400 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | ==pBca1256-K112400== | |
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+ | <pre> | ||
+ | Biobricking of promoter for grpE protein | ||
+ | PCR grpE-F and grpE-R on MG1655 (129 bp, EcoRI/BamHI) | ||
+ | Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) | ||
+ | Product is pBca1256-K112400 | ||
+ | ---------------------------- | ||
+ | cb0001-grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag | ||
+ | cb0002-grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc | ||
+ | </pre> | ||
+ | |||
Latest revision as of 04:08, 24 October 2008
Promoter for grpE gene - Heat Shock and Ultrasound Sensitive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pBca1256-K112400
Biobricking of promoter for grpE protein PCR grpE-F and grpE-R on MG1655 (129 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- cb0001-grpE-F Forward Biobricking cgataGAATTCatgAGATCTccgaggtccttgttgcgaag cb0002-grpE-R Reverse Biobricking cgtagGGATCCcgcattcatctcgctaacttc
Source
cloned from E. coli MG1655 genome