Difference between revisions of "Part:BBa K112001"

 
 
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__NOTOC__
 
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<partinfo>BBa_K112001 short</partinfo>
 
<partinfo>BBa_K112001 short</partinfo>
  
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T4 holin gene without a start codon with a stop codon, in BBb format.
 
 
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This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:
 
This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
 
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
  
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===Information contributed by City of London UK (2021)===
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Part information is collated here to help future users of the BioBrick registry.
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Metadata:
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*'''Group:''' City of London UK 2021
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*'''Author:''' Julian Chen
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*'''Summary:''' Added information collated from existing scientific studies
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It is part of the excision complex necessary for the excision of prophage from the host genome by site-specific recombination at the 'att' site.
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Interacts (via C-terminus) with the integrase (via N-terminus) <ref>'PubMed:12832614'</ref>. Part of the excision complex made of the integrase tetramer, IHF, Fis and Xis <ref>'PubMed:25114241'</ref>. <ref>'Uniprot'. https://www.uniprot.org/uniprot/P03699</ref>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:46, 30 July 2021

Xis from bacteriophage lambda, assembly standard 21

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Julian Chen
  • Summary: Added information collated from existing scientific studies


It is part of the excision complex necessary for the excision of prophage from the host genome by site-specific recombination at the 'att' site.

Interacts (via C-terminus) with the integrase (via N-terminus) [1]. Part of the excision complex made of the integrase tetramer, IHF, Fis and Xis [2]. [3]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ 'PubMed:12832614'
  2. ↑ 'PubMed:25114241'
  3. ↑ 'Uniprot'. https://www.uniprot.org/uniprot/P03699