Difference between revisions of "Part:BBa K2996709"
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Increased expression of luxI enzyme indicates the off-target incidence, and AHL molecules, synthesized by LuxI, will penetrate to the bacteria capable of information storage. | Increased expression of luxI enzyme indicates the off-target incidence, and AHL molecules, synthesized by LuxI, will penetrate to the bacteria capable of information storage. | ||
| + | <center>{{#tag:html|<img style="max-width: 80%" src="https://2019.igem.org/wiki/images/7/73/T--SJTU-BioX-Shanghai--wet_lab-IF1.1.jpg" alt="" />}}</center> | ||
| − | + | <center><b>Figure 1.</b> <i>Gene circuit for transcription activation and information storage based on AHL</i> </center> | |
Latest revision as of 14:48, 21 October 2019
LuxI downstream of activation unit containing lure
LuxI is put downstream of BBa_K2996704, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL). We develop this to record off-target incidence. When the dcas9-sgRNA complex binds to target sequence, RpoA will recruit RNA polymerase, promoter J23117 will be activated. Then, by measuring the biological activity of the downstream reporter gene, we will know the effectiveness of this transcription activation device under different conditions.
Increased expression of luxI enzyme indicates the off-target incidence, and AHL molecules, synthesized by LuxI, will penetrate to the bacteria capable of information storage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 52
Illegal NheI site found at 75 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 743
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 39
- 1000COMPATIBLE WITH RFC[1000]