Difference between revisions of "Part:BBa K3196022"
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<partinfo>BBa_K3196022 short</partinfo> | <partinfo>BBa_K3196022 short</partinfo> | ||
− | FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze | + | FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
− | This is a four section for degrade and transfer lignin part. | + | This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.<sup>[1]</sup> It can help the peptide chain of VP be transferred out cells. [[File:T--HUST--China--2019-FLO10-αproVP.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]] |
− | [[File:T--HUST--China--2019-FLO10-αproVP.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]] | + | |
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+ | <h1>'''Reference'''</h1> | ||
+ | [1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 - 1452; 4 December 2014 ISSN 0001 - 6209; CN 11 - 1995 /Q | ||
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Latest revision as of 10:39, 20 October 2019
AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator
FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.
Characterization
This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.[1] It can help the peptide chain of VP be transferred out cells.Reference
[1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 - 1452; 4 December 2014 ISSN 0001 - 6209; CN 11 - 1995 /Q
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]