Difference between revisions of "Part:BBa K3081116"

 
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<br> The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2)  on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
 
<br> The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2)  on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
  
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<center>
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https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
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</center>
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<center>
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Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
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</center>
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Reference:
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[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:54, 21 October 2019


a kind of 20-bp sgRNA that is designed to be complementary to OriC, the genome replication origin


The 20 bp sgRNA targets to the linker sequence of two DnaA binding boxes (M and R2) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has no inhibition on cell growth. For more detailed information, see BBa_K3081058

T--Peking--box.png

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]


Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]