Difference between revisions of "Part:BBa K3081113"
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<partinfo>BBa_K3081113 short</partinfo> | <partinfo>BBa_K3081113 short</partinfo> | ||
| − | The 20 bp sgRNA targets to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | + | The 20 bp sgRNA targets to the duplex unwinding element (DUE) on <i>E.coli</i> genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see <partinfo>BBa_K3081058</partinfo> |
| + | <center> | ||
| + | https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png | ||
| + | </center> | ||
| + | <center> | ||
| + | Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1] | ||
| + | </center> | ||
| + | |||
| + | |||
| + | Reference: | ||
| + | [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:21, 21 October 2019
a 20bp sequence targeting the DNA unwinding element on E.coli oriC
The 20 bp sgRNA targets to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see BBa_K3081058
Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]
Reference:
[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]