Difference between revisions of "Part:BBa K3081112"
 
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<partinfo>BBa_K3081112 short</partinfo>
 
<partinfo>BBa_K3081112 short</partinfo>
  
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The 20 bp sgRNA targets to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is  
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<br> The 20 bp sgRNA targets to the R4- DnaA box on <i>E.coli</i> genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
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<br>a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
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<center>
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https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
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</center>
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<center>
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Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
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</center>
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Reference:
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[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:20, 21 October 2019


a 20bp sequence targeting the R4(bottom strand) DnaA box on E.coli oriC


The 20 bp sgRNA targets to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058


T--Peking--box.png

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]


Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]