Difference between revisions of "Part:BBa K3093004"
 
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The α-hemolysin secretion pathway has mainly been reported in uropathogenic E. coli. The secretory machinery of this pathway consists of three components: HlyB, an ATP-binding cassette (ABC transporter); HlyD, a membrane fusion protein; and TolC, an outer membrane protein. HlyB and HlyD have been generally considered strain specific proteins, TolC, in contrast, is a component of multiple trans-membrane systems in many microorganisms. Thus, co-expression of HlyB/D is often performed to facilitate the extracellular translocation of proteins utilizing the α-hemolysin secretion pathway during expression in host strains other than uropathogenic E. coli.
 
The α-hemolysin secretion pathway has mainly been reported in uropathogenic E. coli. The secretory machinery of this pathway consists of three components: HlyB, an ATP-binding cassette (ABC transporter); HlyD, a membrane fusion protein; and TolC, an outer membrane protein. HlyB and HlyD have been generally considered strain specific proteins, TolC, in contrast, is a component of multiple trans-membrane systems in many microorganisms. Thus, co-expression of HlyB/D is often performed to facilitate the extracellular translocation of proteins utilizing the α-hemolysin secretion pathway during expression in host strains other than uropathogenic E. coli.
  
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===References===
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[1] Vassilis Koronakis, Peter Stanley, Eva Koronakis, Colin Hughes,The HlyB/HlyD-dependent secretion of toxins by Gran-negative bacteria,FEMS Microbiology Letters,Volume 105, Issues 1–3,1992,Pages 45-53,ISSN 0378-1097.
  
 
===Usage and Biology===
 
===Usage and Biology===
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In order to utilize the less useful shortened cellulose from wastepaper as the raw material to produce bacterial cellulose(BC) in situ, ECUST iGEMers developed “paper transformer”. It was a dual plasmid system(pCL-pCS) including four parts : 1. Cellulose hydrolysis, 2. BC synthesis, 3. Inverter system, 4. Cellobiose response element. We chose E.coli DH5α as the chassis.
 
  
  

Latest revision as of 03:57, 22 October 2019


HlyD

The α-hemolysin secretion pathway has mainly been reported in uropathogenic E. coli. The secretory machinery of this pathway consists of three components: HlyB, an ATP-binding cassette (ABC transporter); HlyD, a membrane fusion protein; and TolC, an outer membrane protein. HlyB and HlyD have been generally considered strain specific proteins, TolC, in contrast, is a component of multiple trans-membrane systems in many microorganisms. Thus, co-expression of HlyB/D is often performed to facilitate the extracellular translocation of proteins utilizing the α-hemolysin secretion pathway during expression in host strains other than uropathogenic E. coli.

References

[1] Vassilis Koronakis, Peter Stanley, Eva Koronakis, Colin Hughes,The HlyB/HlyD-dependent secretion of toxins by Gran-negative bacteria,FEMS Microbiology Letters,Volume 105, Issues 1–3,1992,Pages 45-53,ISSN 0378-1097.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]