Difference between revisions of "Part:BBa K3081107"
| (2 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3081107 short</partinfo> | <partinfo>BBa_K3081107 short</partinfo> | ||
| − | The 20 bp sgRNA targets to the M+(top strand) DnaA box on E.coli genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | + | The 20 bp sgRNA targets to the M+(top strand) DnaA box on <i>E.coli</i> genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> |
| − | + | <center> | |
| − | https://2019.igem.org/wiki/images/ | + | https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png |
| + | </center> | ||
| + | <center> | ||
| + | Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1] | ||
| + | </center> | ||
| + | Reference: | ||
| + | [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:16, 21 October 2019
a 20bp sequence targeting the M (bottom strand) DnaA box on E.coli oriC
The 20 bp sgRNA targets to the M+(top strand) DnaA box on E.coli genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058
Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]
Reference:
[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]