Difference between revisions of "Part:BBa K3037004"

Latest revision as of 22:23, 21 October 2019

Linker

Linker
Function	Linker for fusion proteins
Use in	Escherichia coli
RFC standard	Freiburg RFC25 standard
Backbone	pSB1C3
Submitted by	Team: TU_Dresden 2019

Overview

The TU Dresden team 2019 used this linker to make the fusion proteins (BBa_K3037005) and (BBa_K3037003).

Characterization

This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.

Sequence

This BioBrick is flanked by the RFC25 standard.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 |-
 |'''Backbone'''
-|pSB1C3<br>
+|[https://parts.igem.org/Help:2019_DNA_Distribution pSB1C3<br>]
 |-
 |'''Submitted by'''
@@ Line 20: / Line 20: @@
-=== Overview ===
+== Overview ==
-The TU Dresden team 2019 used this linker in order to make their fusion protein [https://parts.igem.org/Part:BBa_K3037003 BBa_K3037003].
+The TU Dresden team 2019 used this linker to make the fusion proteins [https://parts.igem.org/Part:BBa_K3037005 (BBa_K3037005)] and [https://parts.igem.org/Part:BBa_K3037003 (BBa_K3037003)].
-This BioBrick was used to make the fusion proteins [https://parts.igem.org/Part:BBa_K3037005 (BBa_K3037005)] and [https://parts.igem.org/Part:BBa_K3037003 (BBa_K3037003)]
+== Characterization ==
+This part, encodes for a linker used in between translationally fused proteins. The amino acid composition is "GSAGSAAGSG". The high amounts of glycine and serine residues make it a very flexible linker.
+== Sequence ==
-=== Sequence ===
+This BioBrick is flanked by the RFC25 standard.
 <partinfo>BBa_K3037004 SequenceAndFeatures</partinfo>