Difference between revisions of "Part:BBa K3015006"
| Line 4: | Line 4: | ||
Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part [[Part:BBa_K145001|BBa_K14001]] to improve the applicability. | Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part [[Part:BBa_K145001|BBa_K14001]] to improve the applicability. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 12:33, 19 October 2019
T7 RNA Polymerase (No BbsI/BpiI)
Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part BBa_K14001 to improve the applicability.
Usage and Biology
The T7-polymerase will start transcription from a T7-promotor
We Mutated the following basepairs to delete BbsI/BpiI recognition sites
1) S202 changed from TCT to TCC (bp 604-606)
2) E207 changed from GAA to GAG (bp 619-621)
3) E309 changed from GAA to GAG (bp 925-927)
4) E365 changed from GAA to GAG (bp 1093-1095)
5) K714 changed from AAG to AAA (bp 2140-21429
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]