Difference between revisions of "Part:BBa K2983062"

Latest revision as of 18:52, 21 October 2019

FadX of Trichosanthes kirilowii

This part is an Yarrowia lipolytica codon optimized version of the bifunctional fatty acid conjugase / desaturase (FadX) from Trichosanthes kirilowii (Uniprot Q84UC0).

Usage and Biology

The bifunctional fatty acid conjugase / desaturase FadX (EC: 1.14.19.16) catalyses the conversion of linoleic acid, C18:2 (9Z,12Z) into punicic acid, C18:3 (9Z, 11E, 13Z) (Figure 1).

Figure 1. Conversion of linoleic acid to punicic acid (both incorporated into phosphatidylcholine) by EC: 1.14.19.16.

The Trichosanthes kirilowii (chinese cucumber / chinese snake gourd) FadX (Tk-FadX) [1] is a 44.918 kDa protein of 387 amino acids. It's activity leads to the accumulation of important quantities of punicic acid in the seeds.

When placed them under the control of a constitutive promoter and expressed in the genome of the oleaginous yeast Yarrowia lipolytica, this part leads to the production of punicic acid, a non natural metabolite of Y. lipolytica. For further details, see the Main Page on the Registry of the Tk-FadX expression plasmid (BBa_K2983182) that is able to integrate, upon transformation, into the genome of a Y. lipolytica Po1d stain.

References

[1] Iwabuchi M, Kohno-Murase J, Imamura J. Delta 12-oleate desaturase-related enzymes associated with formation of conjugated trans-delta 11, cis-delta 13 double bonds. J Biol Chem (2003) 278, 4603-4610.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2983062 short</partinfo>
-Conjugated linolenic acids (CLnAs) are synthetized by bifunctional fatty acid conjugase / desaturase (FadX) enzymes from linoleic acid, C18:2 (9Z,12Z).Thus, to convert it into punicic acid, only the presence of a EC: 1.14.19.16 enzyme is necessary (Figure 1).
+This part is an ''Yarrowia lipolytica'' codon optimized version of the bifunctional fatty acid conjugase / desaturase (FadX) from ''Trichosanthes kirilowii'' (Uniprot Q84UC0).
-Two EC: 1.14.19.16 enzymes were described in the literature: one from the chinese cucumber / chinese snake gourd / Trichosanthes kirilowii (Tk-FadX, BBa_K2983062) and another onefrom pomegranate / Punica granatum (Pg-FadX,BBa K2983061)
 ===Usage and Biology===
-This enzyme is used to produce punicic acid from linoleic acid.
-[[File:T--Evry Paris-Saclay--PuA reaction.png|900px|thumb|left| Figure 1:Conversion of linoleic acid to punicic acid  (both incorporated into phosphatidylcholine)]]
+The bifunctional fatty acid conjugase / desaturase FadX (EC: 1.14.19.16) catalyses the conversion of linoleic acid, C18:2 (9Z,12Z) into punicic acid, C18:3 (9Z, 11E, 13Z) (Figure 1).
+[[File:T--Evry Paris-Saclay--PuA reaction.png|900px|thumb|left| Figure 1. Conversion of linoleic acid to punicic acid  (both incorporated into phosphatidylcholine) by EC: 1.14.19.16.]]
+The ''Trichosanthes kirilowii'' (chinese cucumber / chinese snake gourd) FadX (Tk-FadX) [1] is a 44.918 kDa protein of 387 amino acids. It's activity leads to the accumulation of important quantities of punicic acid in the seeds.
+When placed them under the control of a constitutive promoter and expressed in the genome of the oleaginous yeast ''Yarrowia lipolytica'', this part leads to the production of punicic acid, a non natural metabolite of ''Y. lipolytica''. For further details, see the Main Page on the Registry of the Tk-FadX expression plasmid ([[Part:BBa_K2983182|BBa_K2983182]]) that is able to integrate, upon transformation, into the genome of a ''Y. lipolytica'' Po1d stain.
-<!-- Add more about the biology of this part here
+===References===
-===Usage and Biology===
+[1] Iwabuchi M, Kohno-Murase J, Imamura J. Delta 12-oleate desaturase-related enzymes associated with formation of conjugated trans-delta 11, cis-delta 13 double bonds. J Biol Chem (2003) 278, 4603-4610.
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