Difference between revisions of "Part:BBa K3013002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We optimized the sequence for <i>E. coli</i>. | + | We optimized the sequence for <i>E. coli</i>. This part does not contain a start codon, as it is designed for a fusion protein. If you want to use this part alone, consider adding ATG! |
===Source=== | ===Source=== | ||
− | + | We’ve got the sequence of this part from the additional data (https://www.ncbi.nlm.nih.gov/nuccore/EF418615) of the following paper [Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, Wendorff M, Losi A, Gartner W, Jaeger KE: Reporter proteins for in vivo fluorescence without oxygen. Nat Biotechnol. 2007, 25: 443-445. 10.1038/nbt1293.]. | |
===References=== | ===References=== |
Latest revision as of 11:25, 19 October 2019
FbFP BS2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 87
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 255
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We optimized the sequence for E. coli. This part does not contain a start codon, as it is designed for a fusion protein. If you want to use this part alone, consider adding ATG!
Source
We’ve got the sequence of this part from the additional data (https://www.ncbi.nlm.nih.gov/nuccore/EF418615) of the following paper [Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, Wendorff M, Losi A, Gartner W, Jaeger KE: Reporter proteins for in vivo fluorescence without oxygen. Nat Biotechnol. 2007, 25: 443-445. 10.1038/nbt1293.].