Difference between revisions of "Part:BBa K3081013"
 
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<partinfo>BBa_K3081013 short</partinfo>
 
<partinfo>BBa_K3081013 short</partinfo>
  
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation.  
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the duplex unwinding element (DUE) on <i>E.coli</i> genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation.  
  
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<H1>Experiment</H1>
 
For more detailed information, see <partinfo>BBa_K3081058</partinfo>
 
For more detailed information, see <partinfo>BBa_K3081058</partinfo>
  
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https://2019.igem.org/wiki/images/7/75/T--Peking--MR13gif.gif
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（Considering the technical problem, if the reader is interested in our results, welcome to our presentation and poster session to watch the original movie.）
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Reference:
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[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:41, 21 October 2019


pBAD-dCas9-J23119-MR13

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation.

Experiment

For more detailed information, see BBa_K3081058

T--Peking--MR13gif.gif


（Considering the technical problem, if the reader is interested in our results, welcome to our presentation and poster session to watch the original movie.）


Reference:

[1]Wolański M, Donczew R, Zawilakpawlik A, et al. oriC-encoded instructions for the initiation of bacterial chromosome replication.[J]. Frontiers in Microbiology, 2015, 5(735):735.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961