Difference between revisions of "Part:BBa K2912008"
 
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<partinfo>BBa_K2912008 short</partinfo>
 
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This is a single-stranded DNA that can be cyclized by T7 promoter, since it has two sites complementarty to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding protein CAB7 of M. micrantha（Unigene0026534）, through which we can block this essential matabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.
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This is a single-stranded DNA that can be cyclized by the T7 promoter since it has two sites complementary to the T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding protein CAB7 of M. micrantha（Unigene0026534）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.
  
 
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<center><html><img src='https://2019.igem.org/wiki/images/4/44/T--SZU-CHINA--fig._1%EF%BC%884%EF%BC%89.png' style="width:50%;margin:0 auto">
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<center><html><img src='https://2019.igem.org/wiki/images/e/e0/T--SZU-CHINA--fig._14%EF%BC%884%EF%BC%89.png' style="width:50%;margin:0 auto">
 
<center> Fig.1 The Relative Target Gene Expression（Unigene0026534）after Using hairpin siRNA </center></html></center>
 
<center> Fig.1 The Relative Target Gene Expression（Unigene0026534）after Using hairpin siRNA </center></html></center>
 
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 06:39, 19 October 2019


M. micrantha_leaves_ Unigene0026534

This is a single-stranded DNA that can be cyclized by the T7 promoter since it has two sites complementary to the T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding protein CAB7 of M. micrantha（Unigene0026534）, through which we can block this essential metabolic gene expression to kill such invasive weed(illustrated in Fig. 1）.

Fig.1 The Relative Target Gene Expression（Unigene0026534）after Using hairpin siRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]