Difference between revisions of "Part:BBa K121015"

 
(→‎Pressure response)
 
(9 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K121015 short</partinfo>
 
<partinfo>BBa_K121015 short</partinfo>
  
−
pL (cI regulated) GFP reporter
+
pL (lambda cI regulated) + RBS + GFP + TT on pSB6 ([[Part:BBa_I739201]]).
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This biobrick is designed for [http://2008.igem.org/Team:Tokyo_Tech iGEM2008 Tokyo_Tech]
 +
as a weakly pressure inducible promoter.
 +
 +
<!-- Add more about the biology of this part here>
 +
  
 
<!-- -->
 
<!-- -->
Line 12: Line 15:
 
<partinfo>BBa_K121015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K121015 SequenceAndFeatures</partinfo>
  
 +
=== Pressure response===
 +
[[Image:Pressure_response_of_PL.png‎|thumb|400px|center|figure. Pressure response of PL. PL activity increased about 1.4-fold under 30 MPa. ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 03:54, 30 October 2008

pL GFP on pSB6

pL (lambda cI regulated) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Usage and Biology

This biobrick is designed for [http://2008.igem.org/Team:Tokyo_Tech iGEM2008 Tokyo_Tech] as a weakly pressure inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 125
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 812

Pressure response

figure. Pressure response of PL. PL activity increased about 1.4-fold under 30 MPa. ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]