Difference between revisions of "Part:BBa K3105672"
 
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Part to co-express horseradish peroxidase (HRP) and aryl-alcohol oxidase (AAO) in <I>Pichia pastoris</I>. The 2A-peptide will facilitate cleavage of the polypeptide during translation, leading to separate units of HRP and AAO. <br>
 
Part to co-express horseradish peroxidase (HRP) and aryl-alcohol oxidase (AAO) in <I>Pichia pastoris</I>. The 2A-peptide will facilitate cleavage of the polypeptide during translation, leading to separate units of HRP and AAO. <br>
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The part was used in <partinfo>BBa_K3105679</partinfo> for successful expression of HRP and AAO. <br><br>
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3105672 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3105672 SequenceAndFeatures</partinfo>
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Latest revision as of 13:13, 20 October 2019


HRP-2A-AAO

Part to co-express horseradish peroxidase (HRP) and aryl-alcohol oxidase (AAO) in Pichia pastoris. The 2A-peptide will facilitate cleavage of the polypeptide during translation, leading to separate units of HRP and AAO.
The part was used in BBa_K3105679 for successful expression of HRP and AAO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 1275
    Illegal BamHI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
    Illegal AgeI site found at 2422
  • 1000
    COMPATIBLE WITH RFC[1000]