Difference between revisions of "Part:BBa K3105667"

Latest revision as of 01:13, 21 October 2019

Aryl-alcohol oxidase

Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris

In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).

AAO facilitates following reaction:

primary aromatic alcohol + O₂ = aromatic aldehyde + H₂O₂

References:
Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 150
Illegal BamHI site found at 337
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1297
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3105667 short</partinfo>
-Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris
+Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for <I>Pichia pastoris</I>
 In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).
@@ Line 9: / Line 9: @@
 AAO facilitates following reaction:
-primary aromatic alcohol + O2 = aromatic aldehyde + H2O2
+primary aromatic alcohol + O<sub>2</sub> = aromatic aldehyde + H<sub>2</sub>O<sub>2</sub>
 <br><br><br>
 <b>References:</b> <br>