Difference between revisions of "Part:BBa K3317031"
 
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<partinfo>BBa_K3317031 short</partinfo>
 
<partinfo>BBa_K3317031 short</partinfo>
  
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Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) ribosome re-initiation site 1 and ribosome re-initiation site 2 was placed between pduJK and pduN was placed between pduAB and pduJK,this sequence is contained in plasmid pMCY30 generously provided by Dr. Mimi C. Yung
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Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) ''S. enterica'' LT2 (NC_003197.1)An unspecified RBS assumption is found upstream of the pduA protein, ribosome re-initiation site 1 was placed between pduAB and pduJK and ribosome re-initiation site 2 was placed between pduJK and pduN ,this sequence is contained in plasmid pMCY30 generously provided by [https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0685-x#Abs1 Dr. Mimi C. Yung]. The plasmid contains the proteins pduA (<partinfo>K3317008</partinfo>) pduB(<partinfo>K3317009</partinfo>) pduJ
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The plasmid contains the proteins pduA (<partinfo>K3317008</partinfo>) pduB(<partinfo>K3317009</partinfo>) pduJ
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(<partinfo>K3317010</partinfo>) pduK (<partinfo>K3317011</partinfo>) and pduN (<partinfo>K3317012</partinfo>)
 
(<partinfo>K3317010</partinfo>) pduK (<partinfo>K3317011</partinfo>) and pduN (<partinfo>K3317012</partinfo>)
 
   
 
   

Latest revision as of 23:34, 18 October 2019


BMC shell proteins ABJKN from S. enterica LT2

Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1)An unspecified RBS assumption is found upstream of the pduA protein, ribosome re-initiation site 1 was placed between pduAB and pduJK and ribosome re-initiation site 2 was placed between pduJK and pduN ,this sequence is contained in plasmid pMCY30 generously provided by Dr. Mimi C. Yung. The plasmid contains the proteins pduA (BBa_K3317008) pduB(BBa_K3317009) pduJ (BBa_K3317010) pduK (BBa_K3317011) and pduN (BBa_K3317012)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 41
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1761
    Illegal BamHI site found at 1900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 41
    Illegal NgoMIV site found at 1388
    Illegal AgeI site found at 1655
    Illegal AgeI site found at 1691
  • 1000
    COMPATIBLE WITH RFC[1000]