Difference between revisions of "Part:BBa K2984045"
 
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984040">bleomycin resistance</a> gene fused to a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984044">self cleaving peptide</a>.  
 
This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984040">bleomycin resistance</a> gene fused to a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984044">self cleaving peptide</a>.  
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The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every two bleomycin molecules, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:2 to the antibiotic (Hayes et al., 1990). By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme (Lumbreras et al. 1998).
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The self cleaving peptide is designed to cleave after translation of the bleomycin resistance and a desired protein.
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<partinfo>BBa_K2984045 parameters</partinfo>
 
<partinfo>BBa_K2984045 parameters</partinfo>
 
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==References==
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Lumbreras, V., Stevens, D. R., & Purton, S. (1998). Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. The Plant Journal, 14(4), 441-447.
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</li>
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Hayes, J. D., & Wolf, C. R. (1990). Molecular mechanisms of drug resistance. Biochemical Journal, 272(2), 281.
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</ol>

Latest revision as of 21:34, 18 October 2019


bleR+scp - Bleomycin resistance with self cleaving peptide - B1-B1

This part is a part of the Chlamy-HUB-Collection. This part is composed of a bleomycin resistance gene fused to a self cleaving peptide.

The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every two bleomycin molecules, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:2 to the antibiotic (Hayes et al., 1990). By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme (Lumbreras et al. 1998).

The self cleaving peptide is designed to cleave after translation of the bleomycin resistance and a desired protein.

This part was designed to be used with the MoClo standard and has the overhangs B1-B1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 632
  • 1000
    COMPATIBLE WITH RFC[1000]



References

  1. Lumbreras, V., Stevens, D. R., & Purton, S. (1998). Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. The Plant Journal, 14(4), 441-447.
  2. Hayes, J. D., & Wolf, C. R. (1990). Molecular mechanisms of drug resistance. Biochemical Journal, 272(2), 281.