Difference between revisions of "Part:BBa K3105670"
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enhanced green-fluorescent protein codon optimised for <I>Pichia pastoris</I> without start-codon | enhanced green-fluorescent protein codon optimised for <I>Pichia pastoris</I> without start-codon | ||
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===Usage=== | ===Usage=== | ||
eGFP was used as a reporter gene for assessing recombinant protein expression in <I>P. pastoris</I>. For this purpose, C-terminal fusions were created. Examples for this usage are the Parts <partinfo>BBa_K3105677</partinfo> and <partinfo>BBa_K3105680</partinfo>. | eGFP was used as a reporter gene for assessing recombinant protein expression in <I>P. pastoris</I>. For this purpose, C-terminal fusions were created. Examples for this usage are the Parts <partinfo>BBa_K3105677</partinfo> and <partinfo>BBa_K3105680</partinfo>. | ||
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| + | Functionality of this part in <i>P. pastoris</i> was shown in <partinfo>BBa_K3105680</partinfo>. | ||
Latest revision as of 01:15, 21 October 2019
eGFP
enhanced green-fluorescent protein codon optimised for Pichia pastoris without start-codon
Usage
eGFP was used as a reporter gene for assessing recombinant protein expression in P. pastoris. For this purpose, C-terminal fusions were created. Examples for this usage are the Parts BBa_K3105677 and BBa_K3105680.
Functionality of this part in P. pastoris was shown in BBa_K3105680.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]