Difference between revisions of "Part:BBa K2984015"
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| − | This part was designed to be used with the MoClo standard and has | + | This part was designed to be used with the MoClo standard and has B5-B5 overhangs. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2984015 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984015 SequenceAndFeatures</partinfo> | ||
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| + | ==Characterization== | ||
| + | |||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/e/e5/T--Humboldt_Berlin--L0-3xHA-Tag_B5-B5.png" alt="Plate_L0-3xHA-Tag_B5-B5_E.coli" width="500"> | ||
| + | <figcaption>Fig.1 - Successful transformation of L0-3xHA-Tag_B5-B5 into <i>E. coli</i>.</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
| + | |||
| + | ==References== | ||
| + | <ol> | ||
| + | <li> | ||
| + | Wilson, I. A., Niman, H. L., Houghten, R. A., Cherenson, A. R., Connolly, M. L. & Lerner, R. A. ( 1984; ). The structure of an antigenic determinant in a protein. Cell 37, 767-778. | ||
| + | </li> | ||
| + | </ol> | ||
Latest revision as of 20:57, 21 October 2019
3x HA-Tag for protein detection, isolation, purification B5-B5
This part is a part of the Chlamy-HUB-Collection. A popular in vitro purification und detection tag is the Human influenza hemagglutinin (HA) tag (Wilson et al., 1984). It’s a glycoprotein which is required for infectivity of the virus. The tag itself is a small segment of the virus commonly used as an epitope tag in gene expression vectors. Three copies of this tag attached to the desired protein will increase the affinity towards the specific HA-antibodies therefore eliminating the production of protein specific antibodies. The amino acid sequence is YPYDVPDYA. The amino acid sequence was also translated to base triplets in a codon optimized way for C. reinhardtii.
This part was designed to be used with the MoClo standard and has B5-B5 overhangs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
References
- Wilson, I. A., Niman, H. L., Houghten, R. A., Cherenson, A. R., Connolly, M. L. & Lerner, R. A. ( 1984; ). The structure of an antigenic determinant in a protein. Cell 37, 767-778.