Difference between revisions of "Part:BBa K3110009"
 
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<partinfo>BBa_K3110009 short</partinfo>
 
<partinfo>BBa_K3110009 short</partinfo>
  
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L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a weak RBS.
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This construct has L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a weak RBS.
 
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of  
 
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of  
 
L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
 
L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
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===Usage and Biology===
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<partinfo>BBa_K3110009 parameters</partinfo>
 
<partinfo>BBa_K3110009 parameters</partinfo>
 
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<h1>Characterization </h1>
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Due to time constraints we could not characterise this construct.

Latest revision as of 15:24, 21 October 2019


Medium Promoter Weak RBS lldD

This construct has L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and a weak RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 638


Characterization

Due to time constraints we could not characterise this construct.