Difference between revisions of "Part:BBa K3228101"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228101 short</partinfo>
 
<partinfo>BBa_K3228101 short</partinfo>
  
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test
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===Sequence and Features===
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<partinfo>BBa_K3228101 SequenceAndFeatures</partinfo>
  
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<!-- Add more about the biology of this part here
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===Description===
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===Usage and Biology===
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This part has a GFP spaceholder for the gRNA of the crRNA of the CRISPR/cpf1 system. It is used to insert a gRNA for targeted knockouts in the CRISPR/cpf1 system of <a href="https://2019.igem.org/Team:Marburg"> iGEM Marburg 2019.</a>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3228101 SequenceAndFeatures</partinfo>
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===Compability===
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design">Design of the Marburg Collection iGEM Marburg 2018</a>.
  
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===Functional Parameters===
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<partinfo>BBa_K3228101 parameters</partinfo>
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Latest revision as of 17:23, 18 October 2019


pMC_0_3-5_1_crRNA-GFP_dropout

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1186
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1186
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1186
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 242

Description

This part has a GFP spaceholder for the gRNA of the crRNA of the CRISPR/cpf1 system. It is used to insert a gRNA for targeted knockouts in the CRISPR/cpf1 system of iGEM Marburg 2019.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.