Difference between revisions of "Part:BBa K3228100"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228100 short</partinfo>
 
<partinfo>BBa_K3228100 short</partinfo>
  
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test
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===Sequence and Features===
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<partinfo>BBa_K3228100 SequenceAndFeatures</partinfo>
  
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<!-- Add more about the biology of this part here
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===Description===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3228100 SequenceAndFeatures</partinfo>
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This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from <i>Francisella tularensis ssp.</i> novicida, which has been adapted to the MoClo standard <a href="http://2018.igem.org/Team:Marburg/Design">(Ungerer & Pakrasi, 2016)</a>.
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===Compability===
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="https://www.nature.com/articles/srep39681">Design of the Marburg Collection iGEM Marburg 2018</a>.
  
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===Functional Parameters===
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<partinfo>BBa_K3228100 parameters</partinfo>
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Latest revision as of 18:32, 21 October 2019


pMC_0_4_35_cpf1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1713
    Illegal NheI site found at 3433
    Illegal SpeI site found at 3622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 633
    Illegal XbaI site found at 1356
    Illegal SpeI site found at 3622
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part is the coding sequence for the CRISPR-associated endonuclease Cas12a (cpf1) from Francisella tularensis ssp. novicida, which has been adapted to the MoClo standard (Ungerer & Pakrasi, 2016).

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.