Difference between revisions of "Part:BBa K2963015"
 
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<partinfo>BBa_K2963015 short</partinfo>
 
<partinfo>BBa_K2963015 short</partinfo>
  
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According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).
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According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from <i>Corynebacterium glutamicum</i> as the pre-cistron trying to improve the stabilization of the expression of <i>racE</i>(coding racemase enzyme).
  
 
===Usage and Biology===
 
===Usage and Biology===
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The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.
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The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the <i>racE</i> gene, our team used the bicistronic system to further stabilize the expression of the <i>racE</i> gene.
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===Reference===
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Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, <i>Corynebacterium glutamicum</i>." Engineering in Life Sciences (2017).
  
 
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Latest revision as of 11:13, 20 October 2019


BsracE20 - Bicistronic containing RBS20 to stabilize gene expression.

According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).

Usage and Biology

The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team used the bicistronic system to further stabilize the expression of the racE gene.


Reference

Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 998
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]