Difference between revisions of "Part:BBa K2963014"

Latest revision as of 11:12, 20 October 2019

BsracE18 - Bicistronic containing RBS18 to stabilize gene expression.

Usage and Biology

The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the racE gene, we wanted to stabilize the expression of racE gene by using bicistronic system.

Reference

Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 132
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 132
Illegal NheI site found at 999
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 132
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 132
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 132
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.
+The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the <i>racE</i> gene, we wanted to stabilize the expression of <i> racE</i> gene by using bicistronic system.
+===Reference===
+Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, <i>Corynebacterium glutamicum</i>." Engineering in Life Sciences (2017).