Difference between revisions of "Part:BBa K2915300"
| Line 24: | Line 24: | ||
The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3' | The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3' | ||
| − | This NaIII- | + | This NaIII-methylase exists with: |
- with a His-tag and a TEV site | - with a His-tag and a TEV site | ||
Latest revision as of 13:28, 18 October 2019
NaIII-methylase-CBD 6-His-Tag with TEV site
NlaIII is a methylase which is part of an enzymes groupes in part of transferase familly, and tranfert a donor methyl groupe into a an acceptor. This enzyme in our project can allows the binding of the "amylase gene" into the cellulose membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick is in RFC 10 standards with:
The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
This NaIII-methylase exists with:
- with a His-tag and a TEV site