Difference between revisions of "Part:BBa K3239010:Design"

Latest revision as of 07:08, 18 October 2019

pPRM1-MIT1

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 2995
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 2995
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 293
Illegal BglII site found at 3453
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 2995
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 2995
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Parts were fused via Gibson assembly, hence there's no scar.

Source

P. pastoris GS115 and https://www.ncbi.nlm.nih.gov/nuccore/82569479

Registered constructs BBa_K3239000, BBa_K3239004

References

yEGFP3 protein: https://www.ncbi.nlm.nih.gov/protein/82569482

yEGFP2 sequence source: https://www.ncbi.nlm.nih.gov/nuccore/82569479

Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738

Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014

Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053

@@ Line 7: / Line 7: @@
 ===Design Notes===
-Parts were fused via Gibson assembly, hence there were no scars.
+Parts were fused via Gibson assembly, hence there's no scar.
 ===Source===