Difference between revisions of "Part:BBa K2926057"
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− | Mating factor alpha from S. cerevisiae was fused | + | Mating factor alpha from S. cerevisiae was fused N-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy purification of the fusion protein a hexahistidine tag was fused to the C-terminus. |
Latest revision as of 22:58, 17 October 2019
Fusion protein of mating factor alpha, mCherry and pVIII with his-tag
Mating factor alpha from S. cerevisiae was fused N-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy purification of the fusion protein a hexahistidine tag was fused to the C-terminus.
Sequence and Features
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]