Difference between revisions of "Part:BBa K2908681:Design"

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===Design Notes===
 
===Design Notes===
 
The initiation codon ATG can only appear between miRNA sponse and EGFP.<br/>
 
The initiation codon ATG can only appear between miRNA sponse and EGFP.<br/>
−
- The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture.
+
The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture.
 
https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png
 
https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png
  
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===References===
 
===References===
 +
Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26.

Latest revision as of 21:49, 17 October 2019


s(ESR1)p-miR101spe-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initiation codon ATG can only appear between miRNA sponse and EGFP.
The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture. T--CSU_CHINA--ggmodule2.png

Source

We designed it by ourselves and constructed this part with the help of Golden gate.

References

Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26.