Difference between revisions of "Part:BBa K2908675"

Latest revision as of 19:54, 21 October 2019

s(ESR1)p-miR101spe-EGFP

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.
You can see the BBa_K2908681 for detailed information as this part has some bugs.

The physical map of s(ESR1)p-miR101spe-EGFP

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2908675 short</partinfo>
-The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.
+The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.<br/>
-https://static.igem.org/mediawiki/parts/3/3a/T--CSU_CHINA--module2.png
+You can see the BBa_K2908681 for detailed information as this part has some bugs.
+<center>https://2019.igem.org/wiki/images/a/a5/T--CSU_CHINA--2908681.jpg</center><br/>
+<center>The physical map of s(ESR1)p-miR101spe-EGFP</center><br/>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===