Difference between revisions of "Part:BBa K3226020:Design"

Latest revision as of 15:34, 17 October 2019

Add the his tag to BBa_K602008.

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 3396
Illegal suffix found in sequence at 1348
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3396
Illegal SpeI site found at 1349
Illegal PstI site found at 1363
Illegal NotI site found at 1356
Illegal NotI site found at 3402
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3396
Illegal BglII site found at 468
Illegal XhoI site found at 2380
Illegal XhoI site found at 3272
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 3396
Illegal suffix found in sequence at 1349
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 3396
Illegal XbaI site found at 3411
Illegal SpeI site found at 1349
Illegal PstI site found at 1363
Illegal NgoMIV site found at 1142
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We made this parts by using in-fusion cloning. The homologous sequence is needed between insert with vector to do in-fusion cloning. In this way, we took care not to make a mistake in the operation part of the infusion cloning that there is not in the restriction enzyme operation. Please refer to the dedicated page for detailed protocol.

Source

Deinococcus Radiodurans

References

・Tomoyasu Aizawa., (2008)., Ni-NTA affinity column., Protein Science Society archive#019., Protein Science Society of Japan.

・In-Fusion® HD Cloning Kit User Manual., Takara Bio USA, Inc., p.3-4.

@@ Line 15: / Line 15: @@
 ===References===
-Tomoyasu Aizawa., (2008)., Ni-NTA affinity column., Protein Science Society archive#019., Protein Science Society of Japan.
+・Tomoyasu Aizawa., (2008)., Ni-NTA affinity column., Protein Science Society archive#019., Protein Science Society of Japan.
+・In-Fusion® HD Cloning Kit User Manual., Takara Bio USA, Inc., p.3-4.