Difference between revisions of "Part:BBa K2984016"
 
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created.  
 
This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created.  
 
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This part was designed to be used with the MoClo standard and has B5-B5 overhangs.
  
  
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<partinfo>BBa_K2984016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984016 SequenceAndFeatures</partinfo>
  
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==Characterization==
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<html>
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<figure>
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<img src="https://static.igem.org/mediawiki/parts/f/ff/T--Humboldt_Berlin--L0-SP_20_B5-B5.png" alt="Plate_L0-SP_20_B5-B5_E.coli" width="500">
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<figcaption>Fig.1 - Successful transformation of L0-SP_20_B5-B5 into <i>E. coli</i>.</figcaption>
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</figure>
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</html>
  
 
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<partinfo>BBa_K2984016 parameters</partinfo>
 
<partinfo>BBa_K2984016 parameters</partinfo>
 
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==References==
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<ol>
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<li>
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Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.
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</li>
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</ol>

Latest revision as of 20:42, 19 October 2019


SP20 B5-B5 - Glycomodule for enhancement of secretion in C. reinhardtii

This part is a part of the Chlamy-HUB-Collection. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created.

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Plate_L0-SP_20_B5-B5_E.coli
Fig.1 - Successful transformation of L0-SP_20_B5-B5 into E. coli.


References

  1. Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.