Difference between revisions of "Part:BBa K2984001"
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| − | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from C. reinhardtii can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA. | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>.It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from <i>C. reinhardtii</i> can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA. |
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| + | This part was designed to be used with the MoClo standard and has B2-B2 overhangs. | ||
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<partinfo>BBa_K2984001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984001 SequenceAndFeatures</partinfo> | ||
| + | ==Characterization== | ||
| + | |||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/2/28/T--Humboldt_Berlin--L0-GLE_B2-B2.png" alt="Plate_L0-GLE_B2-B2_E.coli" width="500"> | ||
| + | <figcaption>Fig.1 - Successful transformation of L0-GLE_B2-B2 into <i>E. coli</i>.</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
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<partinfo>BBa_K2984001 parameters</partinfo> | <partinfo>BBa_K2984001 parameters</partinfo> | ||
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| + | ==References== | ||
| + | <ol> | ||
| + | <li> | ||
| + | Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224. | ||
| + | </li> | ||
| + | </ol> | ||
Latest revision as of 23:50, 19 October 2019
3'UTR, Secretion signal of gametolysin GLE B2-B2
This vector is a part of the Chlamy-HUB-Collection.It was shown that the putative signal sequence of the metalloprotease gametolysin (GLE) from C. reinhardtii can induce secretion (Ramos-Martinez, Fimognari, & Sakuragi, 2017). The amino acid sequence of it is MSLATRRFGAAAALLVAACVLCTAPAWA.
This part was designed to be used with the MoClo standard and has B2-B2 overhangs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
References
- Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.